Description
Description The MBS human XIAP Enzyme Immunometric Assay (EIA) kit is a complete kit for the determination of XIAP in human cell lysates. Please read the complete kit insert before performing this assay. The kit uses a monoclonal antibody to human XIAP immobilized on a microtiter plate to bind the XIAP in the standards or sample. A recombinant human XIAP Standard is provided in the kit. After a short incubation the excess sample or standard is washed out and a biotinylated monoclonal antibody to human XIAP is added. This antibody binds to the XIAP captured on the plate. After a short incubation the excess antibody is washed out and streptavidin conjugated to Horseradish peroxidase is added, which binds to the biotinylated monoclonal XIAP antibody. Excess conjugate is washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of human XIAP in either standards or samples. For further explanation of the principles nd practices of immunoassays please see the excellent books by Chard1 or Tijssen2